关键词: 大鼠, 针灸, 仰卧位装置固定法, 束缚固定法

Abstract: Objective In order to facilitate the acupuncture and moxibustion experiment in rats, a supine position fixing device which is suitable for the acupuncture and moxibustion operation of acupoints in abdomen and lower limbs of rats was designed on the basis of following the 3R principles. Method Discarded coke bottles were collected and processed into the fixation device according to the predesigned program. By using the natural instince of rats like seeking holes,the rat were bound in supine posture bottle, then the bound rat were stably placed between two fixed bottom plates. The acupoints of the abdomen and lower limbs were fully exposed, and the rats were treated with electroacupuncture. It was compared between the device fixation group and the traditional restraint fixation group from the aspects of the number of operator, the operation time, and the different states of rat undergoing fixation and treatment. Result Compared with the restraint fixation, the fixation of the rat by this device can be completed by only one person, and the fixation time of a single rat is significantly shortened (P<0. 01) . The various conditions of the rat during the fixation and treatment are obvious improve. Conclusion The materials of this device are easy to obtain and easy to modify. Compared with other fixation methods, it has the advantages of simple operation, reliable fixation, saving manpower and time, etc. It is a more applicable method for acupuncture and moxibustion operation on the abdomen and lower limbs of rats, and it provides a new safe and reliable choice for rat fixation in the experiment and is worthy to promote.

Key words: rat, acupuncture, supine device fixation method, restraint fixation